2. Research experience

Molecular and cell biology techniques like cloning, protein expression and purification, PCR, SDS PAGE, Native PAGE, western blotting, immunofluorescence
MicroRNA expression analysis by real time PCR and in situ hybridization
DNA Methylation analysis by bisulfite method and methylation-specific PCR
Radiolabelling oligonucleotide, in vitro binding assay and electrophoretic mobility shift assays, immunoprecipitation assays
Primary cultures and cell line maintenance (both adherent and suspension)
Transfection and transduction in cell lines, generation of stable cell lines (using crispr techniques)
DNA, RNA, microRNA isolation from fresh, frozen or FFPE tissues and cell lines
Generation of lentivirus and lentiviral infections in mammalian cells
siRNAs, shRNAs and RNAi experiments in mammalian cells
Reporter assays such as luciferase, -gal assays
In vivo experimental expertise: Development of cancer models by subcutaneous, orthotopic, and intracardiac implantations in mouse, in-vivo live animal imaging by luminescence, fluorescence

3. Research interest

Monitoring minimal residual disease (MRD) by PCR based on specific Ig/TCR rearrangement identification in childhood ALL patients
Establishing NGS based MRD monitoring and standardizing the workflow for Illumina and Nanopore platforms.
Developing different vectors to facilitate transient/stable cell-line generation to help understanding ALL biology

4. Previous working experience

Postdoctoral Fellow: University of California San Francisco Medical School (Jan 2016 – Jan 2021) Studying the roles of non-coding RNAs and identifying biomarkers for kidney and prostate cancers
PhD student: University of Calcutta (March 2008 – May 2014) Studying anti-proliferative effects and mechanism of action of diallyl disulfides in human leukemic cell-lines


Pubmed link: https://pubmed.ncbi.nlm.nih.gov/?term=pritha+dasgupta

P Kulkarni*, P Dasgupta*, Y Hashimoto, M Shiina, V Shahryari, L Tabatabai, S Yamamura, Y Tanaka, S Saini, R Dahiya, S Majid, “lncRNA TCL6-1 miRNA-155 interaction in the regulation of Src-Akt-EMT network mediated kidney cancer progression and metastasis” Cancer Research, 2021 (*equal contribution) Pubmed

P Dasgupta*, P Kulkarni*, S Majid, Y Hashimoto, M Shiina, N S. Bhat, V Shahryari, L Tabatabai, S Yamamura, Y Tanaka, S Saini, R Dahiya, “LncRNA CDKN2B-AS1/ miR-141/ cyclin D network regulates tumor progression and metastasis of renal cell carcinoma” Cell Death & Disease, 2020 (*equal contribution) PubMed

P Dasgupta*, P Kulkarni*, N S Bhat*, S Majid, M Shiina, V Shahryari, S Yamamura, Y Tanaka, R Kumar, R Dahiya, Y Hashimoto, “Activation of the Erk/MAPK signaling pathway is a driver for cadmium induced prostate cancer” Toxicology and Applied Pharmacology, 2020 (*equal contribution) PubMed

M Imai-Sumida, P Dasgupta, P Kulkarni, M Shiina, Y Hashimoto, V Shahryari, S Majid, Y Tanaka, R Dahiya, S Yamamura, “Genistein represses HOTAIR/chromatin remodeling pathways to suppress kidney cancer” Cellular Physiology and Biochemistry, 2020 PubMed

P Kulkarni , P Dasgupta , N S. Bhat , V Shahryari , M Shiina , Y Hashimoto , S Majid , G Deng , S Saini, Z. L Tabatabai , S Yamamura ,Y Tanaka, R Dahiya, “Elevated miR-182-5p Associates with Renal Cancer Cell Mitotic Arrest through Diminished MALAT-1 Expression” Molecular Cancer Research, 2018 PubMed

P Dasgupta, P Kulkarni, S Majid, V Shahryari, Y Hashimoto, N S Bhat, M Shiina, G Deng, S Saini, S Yamamura, Y Tanaka, R Dahiya,“MicroRNA-203 inhibits long noncoding RNA HOTAIR and regulates tumorigenesis through epithelial-to-mesenchymal transition pathway in renal cell carcinoma” Molecular Cancer Therapeutics, 2018 PubMed