Dwijit Guha Sarkar

1. Introduction

Dwijit Guha Sarkar
Ph.D. in Biomedical Sciences
[email protected]
+91 9163596556

2. Research experience

  • Gene therapy
  • Viral vector (AAV in particular) design and application
  • Mouse models of glioblastoma
  • Cell and molecular biology techniques
  • Protein expression and purification from different systems.

3. Research interest

My current interest at TTCRC involves cell line and organoid model development for Gall Bladder Cancer, characterization and therapeutic strategy design for the same disease using those models.

4. Previous working experience

  • Bose Institute (Oct 2016- June 2020): Postdoctoral Research Associate. Studying human protein kinases to find out hitherto unknown substrates using chemical genetic methods
  • University of Massachusetts Medical School (Sept 2008 –August 2016): PhD student. Adeno-associated viral vector mediated gene therapy of glioblastoma in different mouse models.
  • Calcutta University (August 2007 – August 2008): Project Research Fellow. Preparation and characterization of amino acid coated gold nanoparticles and their biological uses

5. PUBLICATIONS

Pubmed link: https://pubmed.ncbi.nlm.nih.gov/?term=guhasarkar+Dwijit

  1. Volak, A., LeRoy, S. G., Natasan, J. S., Park, D. J., Cheah, P. S., Maus, A., Fitzpatrick, Z., Hudry, E., Pinkham, K., Gandhi, S., Hyman, B. T., Mu, D., GuhaSarkar, D., Stemmer-Rachamimov, A. O., Sena-Esteves, M., Badr, C. E., & Maguire, C. A. (2018). Virus vector-mediated genetic modification of brain tumor stromal cells after intravenous delivery. Journal of neuro-oncology, 139(2), 293–305.
  2. GuhaSarkar D, Neiswender J, Su Q, Gao G, Sena-Esteves M. Intracranial AAV-IFN-β gene therapy eliminates invasive xenograft glioblastoma and improves survival in orthotopic syngeneic murine model. Mol Oncol. 2017 Feb;11(2):180-193.
  3. Golebiowski, D., van der Bom, I., Kwon, C. S., Miller, A. D., Petrosky, K., Bradbury, A. M., Maitland, S., Kühn, A. L., Bishop, N., Curran, E., Silva, N., GuhaSarkar, D., Westmoreland, S. V., Martin, D. R., Gounis, M. J., Asaad, W. F., & Sena-Esteves, M. (2017). Direct Intracranial Injection of AAVrh8 Encoding Monkey β-N-Acetylhexosaminidase Causes Neurotoxicity in the Primate Brain. Human gene therapy, 28(6), 510–522.
  4. GuhaSarkar D, Su Q, Gao G, Sena-Esteves M. Systemic AAV9-IFNβ gene delivery treats highly invasive glioblastoma. Neuro Oncol. 2016 Nov;18(11):1508-1518.
  5. Patra HK, GuhaSarkar D, Dasgupta AK. Multimodal electrophoresis of gold nanoparticles: a real time approach. Anal Chim Acta. 2009 Sep 1;649(1):128-34.